Wednesday, August 10, 2022


Abstract of Current Modifications

  • Clarified the variations between laboratory-based and point-of-care NAATs

A Nucleic Acid Amplification Check, or NAAT, is a sort of viral diagnostic check for SARS-CoV-2, the virus that causes COVID-19. NAATs detect genetic materials (nucleic acids).  NAATs for SARS-CoV-2 particularly determine the RNA (ribonucleic acid) sequences that comprise the genetic materials of the virus.

NAATs for SARS-CoV-2 check specimens from both the higher or decrease respiratory tract. The kind of specimen collected when testing for SARS-CoV-2 relies on the check being carried out and the producer’s directions. See CDC’s Gathering and Dealing with of Medical Specimens for COVID-19 Testing.

The NAAT process works by first amplifying – or making many copies of – the virus’s genetic materials, if any is current in an individual’s specimen. Amplifying these nucleic acids permits NAATs to detect very small quantities of SARS-CoV-2 RNA in a specimen, making these checks extremely delicate for diagnosing COVID-19. In different phrases, NAATs can reliably detect small quantities of SARS-CoV-2 and are unlikely to return a false-negative results of SARS-CoV-2.

NAATs can use many alternative strategies to amplify nucleic acids and detect the virus, together with however not restricted to:

  • Reverse transcription polymerase chain response (RT-PCR)
  • Isothermal amplification together with:
    • Nicking endonuclease amplification response (NEAR)
    • Transcription mediated amplification (TMA)
    • Loop-mediated isothermal amplification (LAMP)
    • Helicase-dependent amplification (HDA)
    • Clustered recurrently interspaced brief palindromic repeats (CRISPR)
    • Strand displacement amplification (SDA)

Because the starting of the COVID-19 pandemic, each the quantity and kinds (strategies and applied sciencesexterior icon) of NAATs approved for emergency use by the U.S. Meals and Drug Administration (FDA) for the detection of SARS-CoV-2 have elevated. The FDA will doubtless authorize extra NAAT strategies sooner or later.

NAATs have been approved to be used in several settings, corresponding to in laboratory services by educated personnel (laboratory-based) or in point-of-care (POC) settings. Some NAATs may even be self-administered at residence or in different non-healthcare places. Some NAATs are thought-about speedy checks which are carried out at or close to the place the place the specimen is collected and might present the end result inside minutes, whereas the time to finish laboratory-based NAATs ranges from lower than an hour to greater than a day. The extent of sensitivity for the detection of SARS-CoV-2 genetic materials in a specimen additionally varies relying on the strategies and software of the NAAT. Sensitivity varies by check, however laboratory-based NAATs usually have increased sensitivity than POC checks or self-administered checks.

As a result of laboratory-based NAATs are thought-about essentially the most delicate checks for detecting SARS-CoV-2, they will also be used to verify the outcomes of decrease sensitivity checks, corresponding to POC NAATs or antigen checks.

If POC NAATs don’t ship presumptive outcomes, they can be utilized for confirmatory testing. If the testing setting doesn’t have the sources or the flexibility to entry laboratory-based NAATs, POC NAATs will also be used for confirmatory testing.  Saliva is a suitable specimen kind for SARS-CoV-2 testing, and a few NAATs have been approved to be used with saliva specimens that present definitive diagnostic and screening outcomes. Nevertheless, saliva specimen high quality may be extremely variable, which may have an effect on the efficiency of the check. CDC recommends accumulating and testing an higher respiratory specimen, corresponding to nasopharyngeal, nasal mid-turbinate, or anterior nasal, when utilizing NAATs for confirmatory testing. In circumstances of discordant check outcomes from various kinds of checks, outcomes from laboratory-based NAATs ought to be prioritized over any POC or self-administered check.

If an individual receives two or extra discordant laboratory-based NAAT outcomes inside a 48-hour interval, the individual ought to contact a healthcare supplier or the native or state well being division for check interpretation and scientific steering on what steps to take.

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