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Data for Laboratories about Coronavirus (COVID-19)


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These sequences are meant for use for the needs of respiratory virus surveillance and analysis. The recipient agrees to make use of them in compliance with all relevant legal guidelines and rules. Each effort has been made to guarantee the accuracy of the sequences, however CDC can’t present any guarantee concerning their accuracy. The recipient can acknowledge the supply of sequences in any oral shows or written publications in regards to the analysis challenge by referring to the Genomics and Diagnostics Group, Virology Surveillance and Prognosis Department, Influenza Division, Nationwide Heart for Immunization and Respiratory Illnesses, Facilities for Illness Management and Prevention, Atlanta, GA, USA.
DDIDNCIRDRespiratoryPanelSARS2FluAB@cdc.gov

Tube 1: Flu SC2 Multiplex Assay: Ahead and Reverse Primers
Identify Description Oligonucleotide Sequence (5’ to three’) Focus/Tube
InfA-F InfA For1 CAA GAC CAA TCY TGT CAC CTC TGA C 3.33 µM
InfA For2 CAA GAC CAA TYC TGT CAC CTY TGA C 3.33 µM
InfA-R InfA Rev1 GCA TTY TGG ACA AAV CGT CTA CG 5.00 µM
InfA Rev2 GCA TTT TGG ATA AAG CGT CTA CG 1.67 µM
InfB-F InfB For TCC TCA AYT CAC TCT TCG AGC G 6.67 µM
InfB-R InfB Rev CGG TGC TCT TGA CCA AAT TGG 6.67 µM
SC2-F SC2 For CTG CAG ATT TGG ATG ATT TCT CC 6.67 µM
SC2-R SC2 Rev CCT TGT GTG GTC TGC ATG AGT TTA G 6.67 µM
RP-F RnaseP For AGA TTT GGA CCT GCG AGC G 6.67 µM
RP-R RnaseP Rev GAG CGG CTG TCT CCA CAA GT 6.67 µM
Tube 2: Flu SC2 Multiplex Assay: Probes
Identify Description Oligonucleotide Sequence (5’>3’) Focus/Tube
InfA-P InfA Probe1 5’-/5FAM/TGC AGT CCT /ZEN/ CGC TCA CTG GGC ACG/3IABkFQ/-3’ 1.67 µM
InfB-P InfB Probe2 5’-/5YakYel/CCA ATT CGA/ZEN/ GCA GCT GAA ACT GCG GTG/3IABkFQ/-3’ 1.67 µM
SC2-P SC2 Probe3 5’-/5TexRd-XN/ATT GCA ACA/TAO/ ATC CAT GAG CAG TGC TGA CTC/3IAbRQSp/-3’ 1.67 µM
RP-P RnaseP Probe4 5’-/5CY5/TTC TGA CCT /TAO/ GAA GGC TCT GCG CG/3IAbRQSp/-3’ 1.67 µM

1 Probe labeled on the 5′ finish with the reporter molecule 6-carboxyfluorescein (FAM), with a ZEN™ quencher between the ninth and tenth nucleotide, and with an Iowa Black FQ quencher (IABkFQ) on the 3′ finish (Built-in DNA Applied sciences, Coralville, IA). InfA probe and primer sequences are similar to InfA sequences within the FDA-cleared CDC Human Influenza Actual-Time RT-PCR Diagnostic Panel (K200370).

2 Probe labeled on the 5′ finish with the Yakima Yellow (YakYel), with a ZEN™ quencher between the ninth and tenth nucleotide, and with an Iowa Black FQ quencher (IABkFQ) on the 3′ finish (Built-in DNA Applied sciences, Coralville, IA). Probe and primer sequences are similar to InfB sequences within the FDA-cleared CDC Human Influenza Virus Actual-Time RT-PCR Detection and Characterization Panel (K080570).

3 Probe labeled on the 5′-end with the Texas Purple-XN (TexRd-XN), with a TAO quencher between the ninth and tenth nucleotide, and with an Iowa Black RQ quencher (IAbRQSp) on the 3′ finish (Built-in DNA Applied sciences, Coralville, IA).

4 Probe labeled on the 5′-end with the CY5 reporter, with a TAO quencher between the ninth and tenth nucleotide, and with an Iowa Black RQ quencher (IAbRQSp) on the 3′ finish (Built-in DNA Applied sciences, Coralville, IA).

 

Observe: Oligonucleotide sequences are topic to future modifications because the SARS-CoV-2 viruses evolve.



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